rabbit anti human cxcr2  (Proteintech)

Journal: Journal of Inflammation Research

Article Title: Mechanistic Study of CD90-Positive Synovial Fibroblasts in the Invasion and Recurrence of Pigmented Villonodular Synovitis

doi: 10.2147/JIR.S549953

Figure Lengend Snippet: Inflammatory cell infiltration is significantly higher in PVNS synovium compared to OA synovium. ( A ) Proportion of all 22 immune infiltration cells based on CIBERSORT in the dataset GSE3698 . ( B ) Boxplots visualize the differences of all 22 immune cells calculated by CIBERSORT in OA, RA and PVNS tissues in GSE3698 . ( C and D ) Immunohistochemistry image for M2 macrophages (CD206; C ) and Neutrophils (CXCR2; D ) in the synovium of the patients with OA and PVNS. Scale bar: 50µm (20X) or 20um (40X). *P < 0.05; **P < 0.01.

Article Snippet: The primary antibodies used were: mouse anti-human CD90 (1:100; Proteintech, China, 66766-1-Ig) and rabbit anti-human PDPN (1:100; Proteintech, China, 11629-1-AP) for immunofluorescence staining; mouse anti-human CD206 (1:100; Proteintech, China, 18704-1-AP) and rabbit anti-human CXCR2 (1:100; Proteintech, China, 20634-1-AP) for immunohistochemical staining.

Techniques: Immunohistochemistry

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Hybrid adherent/suspension cell-based selection strategy leads to discovery of nanobodies against the GPCR CXCR2

Article Snippet: A final competition assay was carried out similarly, titrating either the 10H2 antibody, the CX2_1 nanobody-Fc fusion protein, the IL-8 chemokine, or an irrelevant negative control antibody (tocilizumab) in the presence and absence of a rabbit anti-human CXCR2 polyclonal antibody (Fabgennix, product number CXCR2-201AP) at a 1:50 dilution (133 nM).

Techniques: Selection

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Key resources table

Article Snippet: A final competition assay was carried out similarly, titrating either the 10H2 antibody, the CX2_1 nanobody-Fc fusion protein, the IL-8 chemokine, or an irrelevant negative control antibody (tocilizumab) in the presence and absence of a rabbit anti-human CXCR2 polyclonal antibody (Fabgennix, product number CXCR2-201AP) at a 1:50 dilution (133 nM).

Techniques: Recombinant, Expressing, Modification, Infection, Transfection, Affinity Chromatography, Transduction, Plasmid Preparation, Sequencing, Software

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Hybrid adherent/suspension cell-based selection strategy leads to discovery of nanobodies against the GPCR CXCR2

Article Snippet: A final competition assay was carried out similarly, titrating either the 10H2 antibody, the CX2_1 nanobody-Fc fusion protein, the IL-8 chemokine, or an irrelevant negative control antibody (tocilizumab) in the presence and absence of a rabbit anti-human CXCR2 polyclonal antibody (Fabgennix, product number CXCR2-201AP) at a 1:50 dilution (133 nM).

Techniques: Suspension, Selection

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Key resources table

Article Snippet: A final competition assay was carried out similarly, titrating either the 10H2 antibody, the CX2_1 nanobody-Fc fusion protein, the IL-8 chemokine, or an irrelevant negative control antibody (tocilizumab) in the presence and absence of a rabbit anti-human CXCR2 polyclonal antibody (Fabgennix, product number CXCR2-201AP) at a 1:50 dilution (133 nM).

Techniques: Virus, Recombinant, Expressing, Modification, Infection, Transfection, Affinity Chromatography, Transduction, Plasmid Preparation, Sequencing, Software

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Hybrid adherent/suspension cell-based selection strategy leads to discovery of nanobodies against the GPCR CXCR2

Article Snippet: Rabbit anti-human CXCR2 polyclonal , Fabgennix , Cat#CXCR2-201AP.

Techniques: Suspension, Selection

Journal: Cell Reports Methods

Article Title: Discovery of antibodies targeting multipass transmembrane proteins using a suspension cell-based evolutionary approach

doi: 10.1016/j.crmeth.2023.100429

Figure Lengend Snippet: Key resources table

Article Snippet: Rabbit anti-human CXCR2 polyclonal , Fabgennix , Cat#CXCR2-201AP.

Techniques: Virus, Recombinant, Expressing, Modification, Infection, Transfection, Affinity Chromatography, Transduction, Plasmid Preparation, Sequencing, Software